There are various types of chromatography, including:
1. Gas chromatography (GC): In this method, a volatile mixture of compounds is vaporized and passed through a column filled with a stationary phase (usually a polymer or silica-based material). The compounds in the mixture separate based on their affinity for the stationary phase and their volatility, which affects their retention time in the column. A detector at the end of the column detects the compounds as they elute, producing a chromatogram that can be used to identify the components of the mixture.
2. High-performance liquid chromatography (HPLC): This method is similar to GC but the mixture is separated using a liquid solvent rather than a gas. The stationary phase is a porous material packed in a column, and the sample is injected into the column under high pressure. The components of the sample are separated based on their adsorption, partition, or ion exchange properties. A detector at the end of the column detects the eluting compounds and produces a chromatogram.
3. Ion chromatography (IC): This method involves the separation of ions based on their affinity for a stationary phase that has ion-exchange properties. The sample is injected into the column and the ions in the sample are either attracted or repelled by the stationary phase depending on their charge. The eluting ions are detected using a conductivity detector, producing a chromatogram that can be used for qualitative and quantitative analysis.
4. Affinity chromatography: This method uses the specificity of a ligand to separate a target molecule from a complex mixture. The stationary phase consists of a substrate that has been immobilized on a solid support. The sample containing the target molecule is passed through the column, and the immobilized substrate selectively binds to the target molecule. The remaining components of the sample are washed off and the target molecule is eluted from the column using an appropriate elution buffer.
5. Size exclusion chromatography (SEC): This method separates molecules based on their size or molecular weight. The stationary phase consists of porous beads that allow smaller molecules to penetrate the beads while larger molecules are excluded and elute earlier. The eluting molecules are detected using a UV or refractive index detector, producing a chromatogram that can be used to measure the size or molecular weight of the components in the sample.
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Mix, Match And Mosaic: The Top Interior Design Trends Of 2018
Home Renovation Ideas For 2019
Mix, match and mosaic: the top interior design trends of 2018. Home renovation ideas for 2019. Renovation dossier